Lux primers instrument protocols for using lux primers on the. Clinical chemistry, volume 47, issue 10, 1 october 2001. Thermal cycler supplier agreement pe biosystems and. Smart cycler ii system, capable of performing cycling parameters described in this sop and simultaneous realtime detection for fam, tet, texas red and cy5 fluorophores, cepheid.
The test can provide a highly accurate result in as little as 30 minutes, during the critical period when detection of gbs may potentially reduce the number of infections and deaths to. The laptop connects to the cycler and recognizes it. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smartcycler system software program the software on the computer provided with the smart. Cepheid smartcycler sc1600 starter system, desktop medex supply. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. United states department of agriculture agricultural. The sc2000n31 is an addon block for the cepheid smartcycler ii realtime thermal cycler. Manual extraction, but automated amplification and detection. Smartcycler ii pcr thermalcycler cepheid, sunnyvale, ca capable of performing cycling parameters described below and simultaneous realtime sequence detection for fam, tet, texas red and cy5 dyes. Learn how to use adapter tray retainers in thermal cycler starter kit.
Smartcycler sc1600 starter system includes a processing block, software, operators manual, accessory kit, and desktop computer with cdrw drive and flatpanel monitor. There is a need for new sampleprocessing systems that are quick, reliable, and efficient and that avoid the use of toxic or hazardous chemicals and bulky, expensive equipment. Cepheid is the exclusive owner of the cepheid genexpert dx system software program the software, related docu mentation and physical media, and of all, trade secret, patent, trademark and other intellectual or industrial. Accessory kit includes a minicentrifuge, tube racks, and a cooling block. Manage your pcr protocols using the large, designedforsimplicity, color touchscreen user interface. Computer is windows 2000 with cepheid smart cycler version 2. Rapid and sensitive detection of mycobacterium dna using. Our it staff was able to power on the smart cycler and included laptop. Evaluation of the portable cepheid smartcycler realtime pcr. This modern and compact 96well running pcr easier than ever.
The system includes a smart cycler ii processing block, desktop computer, cdrw drive and flat panel monitor, smart cycler software version 2. Adequate amounts of target dna are produced only through timeconsuming or inefficient manual concentration and lysis methods, or. Cepheid the cepheid smartcycler ii is a modular real time pcr system. Find and purchase cepheid smartcycler products at invitrogen life science technologies. Smartcycler sc1600 td starter system includes a processing block, a laptop computer with cdrw drive, software, an operators manual, a travel case, and an accessory kit. Find out why cepheid s solutions are a top choice for fast, accurate, and easy diagnostic testing. During pcr cycling, optical signals from two sequence specific fluorescent probes within the reaction are monitored in real time.
Surplus property division only tests the units by plugging them up to see if they power on. Cepheid smartcycler td sc1600 starter system, with carry. The optical system of the unit is centered around the icore, a solid state module which does not require warm up, maintenance, or normalization dye. Smartcycler pcr systems, cepheid smartcycler smart cycler ii sc2500n21 sc2500n161 sc2500n121 sc2500n11 sc2500n151 sc2500n191 sc2500n181 sc2500n171 sc2500n1 sc2500n41 sc2500n61 sc2500n51 sc2500n141. System to be distributed by takara in the field and territory shall be an authorized thermal cycler under the terms of the thermal cycling authorization program administered by pe biosystems, a division of pe corporation. For the detection of total cyanobacteria and an internal amplification control for pcr inhibition.
Cepheid smart gbs test for group b streptococcus, run on the smartcyclerr system, receives 510k fda clearance read this article along with other careers information, tips and advice on. The detection of microbial dna in clinical samples by nucleic acid amplification methods is often limited by the low concentration of organisms present in the specimen. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the analyses of nasal swab and serum samples from experimentally infected animals with those obtained from the realtime pcr assay currently in use in the laboratory. Cepheid smartcycler ii thermal cycler automated realtime pcr system 10268. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of. Users guide aircycler g1 installation testing guide aircycler g1 ducted mi. Cepheid launches ce mark certification smart cmvtm in. Cepheid smartcycler ii thermal cycler automated realtime. Used cepheid smart cycler pcr system w statspin m92022. Tb green advantage qpcr premix user manual 051019 takara bio usa, inc.
This allows multiple assays to be run on one or more samples at a time. The smartcycler ii system is calibrated for fam, cy3 or tet or alexafluor532, texas red and cy5 or alexafluor647 q. Smart cycler cold block for smart cycler tubes 20 c adjustable micropipettors 0. Cepheids genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. For the smart cycler, data were analyzed by using the cepheid smart cycler software version 1. Distribution agreement cepheid and takara shuzo co. The t100 thermal cycler is the smart choice for your amplification needs. Cepheid launches ce mark certification smart vzvtm in vitro diagnostic test for smartcycler r platform read this article along with other careers information, tips and advice on biospace.
Type ii cepheids are variable stars which pulsate with periods typically between 1 and 50 days. Cepheid smartcycler ii thermal cycler automated realtime pcr. Manufacturer specifications smartcycler ii td, cepheid please note. Jan 01, 2003 cepheid s smart cycler instrument facilitates the speed and accuracy of the idistrep b test. Find out why cepheids solutions are a top choice for fast, accurate, and easy diagnostic testing. Unit is block only does not include any software or manuals. Cepheid smart cycler pcr system w statspin m92022 rp. Cepheid smartcycler ii td pcr manufacturer specifications.
Average time to results is 2040 minutes, less than half the time of a 96well plate instrument. Cepheid smartcycler system software license agreement license grant. Cepheid smart cycler ii td realtime pcr system govdeals. Cepheid smartcycler td sc1600 starter system, with carry case. Description of the cepheid smartcycler starter system smartcycler td sc1600. Thermal cycler spectral calibration instructions why is spectral calibration necessary.
Manual extraction, but automated amplification and. Description of the cepheid smartcycler starter system, desktop. Cepheid launches ce mark certification smart vzvtm in vitro. Systems can optimize pcr denaturation, annealing and extension temperatures, and times in a single run. Cepheid smart cycler real time pcr advanced search keywords. Phytoxigene cyanodtec cyanobacteria test diagnostic.
Evaluation of the portable cepheid smartcycler realtime. Cepheid smartcycler ii biorad mj opticon 2 realtime instruments not supported abi prism 7000 abi prism 7300 abi prism 7500 abi prism 7900 cepheid smartcycler emission spectra of cal fluor and quasar dyes. Cepheid s genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. Introduction the tb green advantage qpcr premix is a reagent supplied at 2x concentration, consisting of a fulllength taq polymerase with a hotstart taq antibody and tb green dye, that has been specially designed for realtime pcr using the dye intercalator method. Adequate amounts of target dna are produced only through timeconsuming or inefficient manual concentration and lysis methods, or expensive, dedicated equipment. The accessory kit features a minicentrifuge, tube racks, and a cooling rack specifically designed to accommodate smart cycler reaction tubes. Two smartcycler units can process an equal or greater number of samples per day than a 96well instrument.
Cepheid smart cycler system authorized thermal cycler 1 serial. Jan 12, 2017 cepheid smartcycler ii thermal cycler automated realtime pcr system 10268. Apr 11, 2006 the cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled icore intelligent coolingheating optical reaction module. C m y cm my cy cmy k lightcycler 480 instrument operators manual software version 1. It is a molded plastic housing containing an immobilized submicrometer filter, lysis beads manual extras windows 70% similar ships free to lower 48 states a business address or your local fed ex ground shipping center. Csu surplus property is selling a cepheid smart cycler ii td realtime pcr system previously used in a university setting. Cepheid smartcycler sc1600 starter system, desktop medex.
Sluis ceo, cepheid cfo, cepheid 4085414191 4085414191 john. Cepheid accessory for smartcycler pcr systems, cepheid. The smart cycler settings consisted of a primary curve analysis with a manual threshold setting of 10, background subtraction turned on, boxcar average set to five cycles, background minimum cycle set to 5, and background maximum cycle set to 45. Cepheid smartcycler, configuration setup computer block 1 block 2 usb 1. Cepheid enabling access to molecular diagnostic testing. The cepheid smart cycler dx system is an integrated nucleic acid amplification and detection instrument system based on the proprietary microprocessorcontrolled icore module. As many as six of these blocks can be added to a single smartcycler unit, providing up to 96 independently controllable sites for read more. Used cepheid smart cycler real time pcr 2024671 real. The test can provide a highly accurate result in as little as 30 minutes, during the critical period when detection of gbs may potentially reduce the number of infections and deaths to newborns caused by the bacterium. The smart cycler software ensures ease of use for the sixteen independently controlled icore modules found on the smart cycler processing block. In order to successfully use duallabeled probes containing cal fluor andor quasar dyes in realtime, quantitative pcr qpcr multiplexed assays, certain realtime pcr instruments must first be calibrated to recognize the pure dye spectra. Phytoxigene cyanodtec cyanobacteria test diagnostic technology. Cepheids smart cycler instrument facilitates the speed and accuracy of the idistrep b test.
Key features sixteen icore intelligent coolingheating optical reaction modules function as independent thermal cyclerfluorometers. The cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled icore intelligent coolingheating optical reaction module. This block upgrades any existing smartcycler to accommodate an addit. Each reaction site can be programmed and run independently.
This block upgrades any existing smartcycler to accommodate an additional 16 reaction sites for real time pcr analysis. We designed, optimized, and extensively tested several sensitive and specific realtime pcr assays for rapid detection of both smallpox and pan orthopox virus dnas. Cal fluor and uasar yes thermal cycler spectral calibration. If protocol optimization is required, determine your optimal. It is a molded plastic housing containing an immobilized submicrometer filter, lysis beads cepheid smart cycler ii rtpcr model sc2 with 16 well block, including computer with software. Citeseerx document details isaac councill, lee giles, pradeep teregowda. Smallpox and panorthopox virus detection by realtime 3. Lux primers instrument protocols thermo fisher scientific. The cepheid onepiece valve body combines filtration, wash, and lysis functions. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the analyses. Oct 01, 2001 adequate amounts of target dna are produced only through timeconsuming or inefficient manual concentration and lysis methods, or expensive, dedicated equipment.
Vic, hex, and joe are very similar to tet and therefore may work with our calibration, but we cannot guarantee their performance since the base it is attached. Dec 11, 2006 cepheid smart gbs test for group b streptococcus, run on the smartcycler r system, receives 510k fda clearance read this article along with other careers information, tips and advice on biospace. Cepheid smart cycler sc1 realtime 16 site pcr thermal. The smartcycler is a realtime pcr instrument with 16, 34, 48, 64, 80 or 96 independent reaction sites.
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